Membrane protein orientation and refinement using a knowledge-based statistical potential.

ABSTRACT:

Background

Recent increases in the number of deposited membrane protein crystal structures necessitate the use of automated computational tools to position them within the lipid bilayer. Identifying the correct orientation allows us to study the complex relationship between sequence, structure and the lipid environment, which is otherwise challenging to investigate using experimental techniques due to the difficulty in crystallising membrane proteins embedded within intact membranes.

Results

We have developed a knowledge-based membrane potential, calculated by the statistical analysis of transmembrane protein structures, coupled with a combination of genetic and direct search algorithms, and demonstrate its use in positioning proteins in membranes, refinement of membrane pro

SUBMITTER: Nugent T 

PROVIDER: S-EPMC3852961 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature