Comprehensive analysis of RNA-seq data reveals the complexity of the transcriptome in Brassica rapa.

ABSTRACT:

Background

The species Brassica rapa (2n=20, AA) is an important vegetable and oilseed crop, and serves as an excellent model for genomic and evolutionary research in Brassica species. With the availability of whole genome sequence of B. rapa, it is essential to further determine the activity of all functional elements of the B. rapa genome and explore the transcriptome on a genome-wide scale. Here, RNA-seq data was employed to provide a genome-wide transcriptional landscape and characterization of the annotated and novel transcripts and alternative splicing events across tissues.

Results

RNA-seq reads were generated using the Illumina platform from six different tissues (root, stem, leaf, flower, silique and callus) of the B. rapa accession Chiifu-401-42, the same line used

SUBMITTER: Tong C 

PROVIDER: S-EPMC3853194 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature