Pleiotropic effects of cancer cells' secreted factors on human stromal (mesenchymal) stem cells.

ABSTRACT:

Introduction

Studying cancer tumors' microenvironment may reveal a novel role in driving cancer progression and metastasis. The biological interaction between stromal (mesenchymal) stem cells (MSCs) and cancer cells remains incompletely understood. Herein, we investigated the effects of tumor cells' secreted factors as represented by a panel of human cancer cell lines (breast (MCF7 and MDA-MB-231); prostate (PC-3); lung (NCI-H522); colon (HT-29) and head & neck (FaDu)) on the biological characteristics of MSCs.

Methods

Morphological changes were assessed using fluorescence microscopy. Changes in gene expression were assessed using Agilent microarray and qRT-PCR. GeneSpring 12.1 and DAVID tools were used for bioinformatic and signaling pathway analyses. Cell migration was ass

SUBMITTER: Al-toub M 

PROVIDER: S-EPMC3854757 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature