Single color FRET based measurements of conformational changes of proteins resulting from translocation inside cells.

ABSTRACT: Translocation of proteins to different parts of the cell is necessary for many cellular mechanisms as a means for regulation and a variety of other functions. Identifying how these proteins undergo conformational changes or interact with various partners during these events is critical to understanding how these mechanisms are executed. A protocol is presented that identifies conformational changes in a protein that occur during translocation while overcoming challenges in extracting distance information in very different environments of a living cell. Only two samples are required to be prepared and are observed with one optical setup. Live-cell FRET imaging has been applied to identify conformational changes between two native cysteines in Bax, a member of the Bcl-2 family of proteins th

SUBMITTER: Gahl RF 

PROVIDER: S-EPMC3855077 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature