Characterisation and in silico analysis of interleukin-4 cDNA of nilgai (Boselaphus tragocamelus) and Indian buffalo (Bubalus bubalis).

ABSTRACT: Interleukin-4 (IL-4) produced from Th2 cells modulates both innate and adaptive immune responses. It is a common belief that wild animals possess better immunity against diseases than domestic and laboratory animals; however, the immune system of wild animals is not fully explored yet. Therefore, a comparative study was designed to explore the wildlife immunity through characterisation of IL-4 cDNA of nilgai, a wild ruminant, and Indian buffalo, a domestic ruminant. Total RNA was extracted from peripheral blood mononuclear cells of nilgai and Indian buffalo and reverse transcribed into cDNA. Respective cDNA was further cloned and sequenced. Sequences were analysed in silico and compared with their homologues available at GenBank. The deduced 135 amino acid protein of nilgai IL-4 is 95.6% s

SUBMITTER: Saini M 

PROVIDER: S-EPMC3856167 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature