Histone H3 and H4 N-terminal tails in nucleosome arrays at cellular concentrations probed by magic angle spinning NMR spectroscopy.

ABSTRACT: Chromatin is a supramolecular assembly of DNA and histone proteins, organized into nucleosome repeat units. The dynamics of chromatin organization regulates DNA accessibility to eukaryotic transcription and DNA repair complexes. Yet, the structural and dynamic properties of chromatin at high concentrations characteristic of the cellular environment (>∼200 mg/mL) are largely unexplored at the molecular level. Here, we apply MAS NMR to directly probe the dynamic histone protein regions in (13)C,(15)N-enriched recombinant nucleosome arrays at cellular chromatin concentrations and conditions designed to emulate distinct states of DNA condensation, with focus on the flexible H3 and H4 N-terminal tails which mediate chromatin compaction. 2D (1)H-(13)C and (1)H-(15)N spectra reveal numerous corre

SUBMITTER: Gao M 

PROVIDER: S-EPMC3856215 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature