High-density lipoprotein endocytosis in endothelial cells.

ABSTRACT:

Aim

To describe the way stations of high-density lipoprotein (HDL) uptake and its lipid exchange in endothelial cells in vitro and in vivo.

Methods

A combination of fluorescence microscopy using novel fluorescent cholesterol surrogates and electron microscopy was used to analyze HDL endocytosis in great detail in primary human endothelial cells. Further, HDL uptake was quantified using radio-labeled HDL particles. To validate the in vitro findings mice were injected with fluorescently labeled HDL and particle uptake in the liver was analyzed using fluorescence microscopy.

Results

HDL uptake occurred via clathrin-coated pits, tubular endosomes and multivesicular bodies in human umbilical vein endothelial cells. During uptake and resecretion, HDL-derived cholesterol was

SUBMITTER: Fruhwurth S 

PROVIDER: S-EPMC3856308 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature