Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production.

ABSTRACT:

Background

Microbial production of biofuels requires robust cell growth and metabolism under tough conditions. Conventionally, such tolerance phenotypes were engineered through evolutionary engineering using the principle of "Mutagenesis followed-by Selection". The iterative rounds of mutagenesis-selection and frequent manual interventions resulted in discontinuous and inefficient strain improvement processes. This work aimed to develop a more continuous and efficient evolutionary engineering method termed as "Genome Replication Engineering Assisted Continuous Evolution" (GREACE) using "Mutagenesis coupled-with Selection" as its core principle.

Results

The core design of GREACE is to introduce an in vivo continuous mutagenesis mechanism into microbial cells by introducing a

SUBMITTER: Luan G 

PROVIDER: S-EPMC3856464 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature