Bioorthogonal approach to identify unsuspected drug targets in live cells.

ABSTRACT: A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets.

SUBMITTER: Yang KS 

PROVIDER: S-EPMC3856564 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature