Genome-wide analysis of a Wnt1-regulated transcriptional network implicates neurodegenerative pathways.

ABSTRACT: Wnt proteins are critical to mammalian brain development and function. The canonical Wnt signaling pathway involves the stabilization and nuclear translocation of β-catenin; however, Wnt also signals through alternative, noncanonical pathways. To gain a systems-level, genome-wide view of Wnt signaling, we analyzed Wnt1-stimulated changes in gene expression by transcriptional microarray analysis in cultured human neural progenitor (hNP) cells at multiple time points over a 72-hour time course. We observed a widespread oscillatory-like pattern of changes in gene expression, involving components of both the canonical and the noncanonical Wnt signaling pathways. A higher-order, systems-level analysis that combined independent component analysis, waveform analysis, and mutual information-based

SUBMITTER: Wexler EM 

PROVIDER: S-EPMC3856943 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature