Oxidatively modified proteins as plasma biomarkers in breast cancer.

ABSTRACT:

Background

Post-translational protein modifications (PTMs) are increased in breast tumors.

Objective

We explored whether PTMs on proteins secreted by the breast could be detected in plasma and potentially used for the early detection of breast cancer.

Methods

We used a custom ELISA microarray platform to measure 4-hydroxynonenal (HNE), glutathione (GSH), nitrotyrosine and halotyrosine adducts in 27 secreted proteins, for a total of 108 candidate biomarkers. Two independent sets of human plasma samples were measured, for a total of 160 samples. The results were analyzed for consistent cancer-associated changes across the two sample sets. Plasma samples for both cases and benign controls were collected at the time of tissue diagnosis after referral from a positive scree

SUBMITTER: Jin H 

PROVIDER: S-EPMC3856946 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature