Transcriptome and small RNA deep sequencing reveals deregulation of miRNA biogenesis in human glioma.

ABSTRACT: Altered expression of oncogenic and tumour-suppressing microRNAs (miRNAs) is widely associated with tumourigenesis. However, the regulatory mechanisms underlying these alterations are poorly understood. We sought to shed light on the deregulation of miRNA biogenesis promoting the aberrant miRNA expression profiles identified in these tumours. Using sequencing technology to perform both whole-transcriptome and small RNA sequencing of glioma patient samples, we examined precursor and mature miRNAs to directly evaluate the miRNA maturation process, and examined expression profiles for genes involved in the major steps of miRNA biogenesis. We found that ratios of mature to precursor forms of a large number of miRNAs increased with the progression from normal brain to low-grade and then to high

SUBMITTER: Moore LM 

PROVIDER: S-EPMC3857031 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature