Absolute quantitation of immunoglobulin G and its glycoforms using multiple reaction monitoring.

ABSTRACT: Studies aimed toward glycan biomarker discovery have focused on glycan characterization by the global profiling of released glycans. Site-specific glycosylation analysis is less developed but may provide new types of biomarkers with higher sensitivity and specificity. Quantitation of peptide-conjugated glycans directly facilitates the differential analysis of distinct glycoforms associated with specific proteins at distinct sites. We have developed a method using MRM to monitor protein glycosylation normalized to absolute protein concentrations to examine quantitative changes in glycosylation at a site-specific level. This new approach provides information regarding both the absolute amount of protein and the site-specific glycosylation profile and will thus be useful to determine if alter

SUBMITTER: Hong Q 

PROVIDER: S-EPMC3857154 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature