Improving embryonic stem cell expansion through the combination of perfusion and Bioprocess model design.

ABSTRACT:

Background

High proliferative and differentiation capacity renders embryonic stem cells (ESCs) a promising cell source for tissue engineering and cell-based therapies. Harnessing their potential, however, requires well-designed, efficient and reproducible expansion and differentiation protocols as well as avoiding hazardous by-products, such as teratoma formation. Traditional, standard culture methodologies are fragmented and limited in their fed-batch feeding strategies that afford a sub-optimal environment for cellular metabolism. Herein, we investigate the impact of metabolic stress as a result of inefficient feeding utilizing a novel perfusion bioreactor and a mathematical model to achieve bioprocess improvement.

Methodology/principal findings

To characterize nutritional

SUBMITTER: Yeo D 

PROVIDER: S-EPMC3858261 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature