Locus-specific editing of histone modifications at endogenous enhancers.

ABSTRACT: Mammalian gene regulation is dependent on tissue-specific enhancers that can act across large distances to influence transcriptional activity. Mapping experiments have identified hundreds of thousands of putative enhancers whose functionality is supported by cell type-specific chromatin signatures and striking enrichments for disease-associated sequence variants. However, these studies did not address the in vivo functions of the putative elements or their chromatin states and did not determine which genes, if any, a given enhancer regulates. Here we present a strategy to investigate endogenous regulatory elements by selectively altering their chromatin state using programmable reagents. Transcription activator-like (TAL) effector repeat domains fused to the LSD1 histone demethylase effici

SUBMITTER: Mendenhall EM 

PROVIDER: S-EPMC3858395 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature