Whole-brain circuit dissection in free-moving animals reveals cell-specific mesocorticolimbic networks.

ABSTRACT: The ability to map the functional connectivity of discrete cell types in the intact mammalian brain during behavior is crucial for advancing our understanding of brain function in normal and disease states. We combined designer receptor exclusively activated by designer drug (DREADD) technology and behavioral imaging with μPET and [18F]fluorodeoxyglucose (FDG) to generate whole-brain metabolic maps of cell-specific functional circuits during the awake, freely moving state. We have termed this approach DREADD-assisted metabolic mapping (DREAMM) and documented its ability in rats to map whole-brain functional anatomy. We applied this strategy to evaluating changes in the brain associated with inhibition of prodynorphin-expressing (Pdyn-expressing) and of proenkephalin-expressing (Penk-expres

SUBMITTER: Michaelides M 

PROVIDER: S-EPMC3859392 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature