Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.

ABSTRACT: Autoantibodies against cytokines, chemokines, and growth factors inhibit normal immunity and are implicated in inflammatory autoimmune disease and diseases of immune deficiency. In an effort to evaluate serum from autoimmune and immunodeficient patients for Abs against cytokines, chemokines, and growth factors in a high-throughput and unbiased manner, we constructed a multiplex protein microarray for detection of serum factor-binding Abs and used the microarray to detect autoantibody targets in SLE. We designed a nitrocellulose-surface microarray containing human cytokines, chemokines, and other circulating proteins and demonstrated that the array permitted specific detection of serum factor-binding probes. We used the arrays to detect previously described autoantibodies against cytokines

SUBMITTER: Price JV 

PROVIDER: S-EPMC3859403 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature