Next generation sequencing analysis of human platelet PolyA+ mRNAs and rRNA-depleted total RNA.

ABSTRACT:

Background

Platelets are small anucleate cells circulating in the blood vessels where they play a key role in hemostasis and thrombosis. Here, we compared platelet RNA-Seq results obtained from polyA+ mRNA and rRNA-depleted total RNA.

Materials and methods

We used purified, CD45 depleted, human blood platelets collected by apheresis from three male and one female healthy blood donors. The Illumina HiSeq 2000 platform was employed to sequence cDNA converted either from oligo(dT) isolated polyA+ RNA or from rRNA-depleted total RNA. The reads were aligned to the GRCh37 reference assembly with the TopHat/Cufflinks alignment package using Ensembl annotations. A de novo assembly of the platelet transcriptome using the Trinity software package and RSEM was also performed. The bioin

SUBMITTER: Kissopoulou A 

PROVIDER: S-EPMC3859545 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature