Global transcriptome sequencing using the Illumina platform and the development of EST-SSR markers in autotetraploid alfalfa.

ABSTRACT:

Background

Alfalfa is the most widely cultivated forage legume and one of the most economically valuable crops in the world. The large size and complexity of the alfalfa genome has delayed the development of genomic resources for alfalfa research. Second-generation Illumina transcriptome sequencing is an efficient method for generating a global transcriptome sequence dataset for gene discovery and molecular marker development in alfalfa.

Methodology/principal findings

More than 28 million sequencing reads (5.64 Gb of clean nucleotides) were generated by Illumina paired-end sequencing from 15 different alfalfa tissue samples. In total, 40,433 unigenes with an average length of 803 bp were obtained by de novo assembly. Based on a sequence similarity search of known proteins, a

SUBMITTER: Liu Z 

PROVIDER: S-EPMC3861513 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature