Evaluation of assembly strategies using RNA-seq data associated with grain development of wheat (Triticum aestivum L.).

ABSTRACT: Wheat (Triticum aestivum L.) is one of the most important crops cultivated worldwide. Identifying the complete transcriptome of wheat grain could serve as foundation for further study of wheat seed development. However, the relatively large size and the polyploid complexity of the genome have been substantial barriers to molecular genetics and transcriptome analysis of wheat. Alternatively, RNA sequencing has provided some useful information about wheat genes. However, because of the large number of short reads generated by RNA sequencing, factors that are crucial to transcriptome assembly, including software, candidate parameters and assembly strategies, need to be optimized and evaluated for wheat data. In the present study, four cDNA libraries associated with wheat grain development wer

SUBMITTER: Li HZ 

PROVIDER: S-EPMC3861526 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature