Large-scale top-down proteomics of the human proteome: membrane proteins, mitochondria, and senescence.

ABSTRACT: Top-down proteomics is emerging as a viable method for the routine identification of hundreds to thousands of proteins. In this work we report the largest top-down study to date, with the identification of 1,220 proteins from the transformed human cell line H1299 at a false discovery rate of 1%. Multiple separation strategies were utilized, including the focused isolation of mitochondria, resulting in significantly improved proteome coverage relative to previous work. In all, 347 mitochondrial proteins were identified, including ~50% of the mitochondrial proteome below 30 kDa and over 75% of the subunits constituting the large complexes of oxidative phosphorylation. Three hundred of the identified proteins were found to be integral membrane proteins containing between 1 and 12 transmembran

SUBMITTER: Catherman AD 

PROVIDER: S-EPMC3861700 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature