SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.

ABSTRACT: Proteomics has been successfully used for cell culture on dishes, but more complex cellular systems have proven to be challenging and so far poorly approached with proteomics. Because of the complexity of the angiogenic program, we still do not have a complete understanding of the molecular mechanisms involved in this process, and there have been no in depth quantitative proteomic studies. Plating endothelial cells on matrigel recapitulates aspects of vessel growth, and here we investigate this mechanism by using a spike-in SILAC quantitative proteomic approach. By comparing proteomic changes in primary human endothelial cells morphogenesis on matrigel to general adhesion mechanisms in cells spreading on culture dish, we pinpoint pathways and proteins modulated by endothelial cells. The ce

SUBMITTER: Zanivan S 

PROVIDER: S-EPMC3861710 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature