An increase in phosphorylation and truncation of crystallin with the progression of cataracts.

ABSTRACT:

Background

Cataracts are the leading cause of blindness worldwide; however, there is no evidence regarding the direct formation of cataracts. At present, there is no treatment method other than surgery to prevent the formation or progression of cataracts.

Objective

Understanding the protein changes during various stages of cataracts might help realize the mechanism of the formation and progression of cataracts.

Methods

Lens materials were collected from cataract surgery. Cataracts were classified according to lens opacity using the gradation of the Lens Opacities Classification System. Lens proteins were separated by 2-dimensional polyacrylamide gel electrophoresis. Protein spots were visualized by Coomassie blue staining, and expression patterns were analyzed. Protei

SUBMITTER: Lin HJ 

PROVIDER: S-EPMC3862202 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature