Damage to the Drosophila follicle cell epithelium produces "false clones" with apparent polarity phenotypes.

ABSTRACT: The Drosophila follicular epithelium, which surrounds developing egg chambers, is a well-established model for studying epithelial polarity because it is continuously generated from adult stem cells, making it easy to generate homozygous mutant clones in a heterozygous background. Mutant clones are usually marked by the loss of Green Fluorescent Protein (GFP) expression, which distinguishes them from their green, wild-type neighbours. Here we report that damage to the epithelium during dissection can produce groups of GFP-negative cells that resemble mutant clones. Furthermore, several polarity factors, such as aPKC and Discs large, are not localised in these damage-induced false clones. This phenotype is identical to that reported for several mutants, including ampk and Dystroglycan mutan

SUBMITTER: Haack T 

PROVIDER: S-EPMC3863415 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature