Simultaneous detection of cell-secreted TNF-α and IFN-γ using micropatterned aptamer-modified electrodes.

ABSTRACT: Cellular production of such cytokines as interferon (IFN)-γ and tumor necrosis factor (TNF)-α is used to determine disease-specific immune responses and may be used to diagnose infectious diseases such as tuberculosis. In this paper, we describe the development of micropatterned electrodes functionalized with electroactive aptamers for multiplexed detection of immune-cell-produced cytokines. A sequence of electrode deprotection and aptamer incubation steps were used to assemble anti-IFN-γ DNA aptamers and anti-TNF-α RNA aptamers on individually addressable half-ring electrodes. Aptamer molecules were thiolated for assembly on gold and were functionalized with methylene blue redox reporter for electrochemical signal transduction. Specificity of individual sensors to the correct cytokine spe

SUBMITTER: Liu Y 

PROVIDER: S-EPMC3863689 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature