Folding of 16S rRNA in a signal-producing structure for the detection of bacteria.

ABSTRACT: Sixty-four DNA strands hybridize to 16S rRNA to form 32 deoxyribozyme catalytic cores that produce a fluorescent signal. The approach allows detection of 0.6 pM 16S rRNA, or about 3×10(4) bacterial cells in a PCR-free format.

SUBMITTER: Gerasimova YV 

PROVIDER: S-EPMC3863991 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature