In vivo three-photon microscopy of subcortical structures within an intact mouse brain.

ABSTRACT: Two-photon fluorescence microscopy (2PM)¹ enables scientists in various fields including neuroscience^2,3, embryology⁴, and oncology⁵ to visualize in vivo and ex vivo tissue morphology and physiology at a cellular level deep within scattering tissue. However, tissue scattering limits the maximum imaging depth of 2PM within the mouse brain to the cortical layer, and imaging subcortical structures currently requires the removal of overlying brain tissue³ or the insertion of optical probes^6,7. Here we demonstrate non-invasive, high resolution, in vivo imaging of subcortical structures within an intact mouse brain using three-photon fluorescence microscopy (3PM) at a spectral excitation window of 1,700 nm. Va

SUBMITTER: Horton NG 

PROVIDER: S-EPMC3864872 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature