Finding markers that make a difference: DNA pooling and SNP-arrays identify population informative markers for genetic stock identification.

ABSTRACT: Genetic stock identification (GSI) using molecular markers is an important tool for management of migratory species. Here, we tested a cost-effective alternative to individual genotyping, known as allelotyping, for identification of highly informative SNPs for accurate genetic stock identification. We estimated allele frequencies of 2880 SNPs from DNA pools of 23 Atlantic salmon populations using Illumina SNP-chip. We evaluated the performance of four common strategies (global F ST, pairwise F ST, Delta and outlier approach) for selection of the most informative set of SNPs and tested their effectiveness for GSI compared to random sets of SNP and microsatellite markers. For the majority of cases, SNPs selected using the outlier approach performed best followed by pairwise F ST and Delta me

SUBMITTER: Ozerov M 

PROVIDER: S-EPMC3864958 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature