Reference genes selection for quantitative real-time PCR using RankAggreg method in different tissues of Capra hircus.

ABSTRACT: Identification of reference genes with stable levels of gene expression is an important prerequisite for obtaining reliable results in analysis of gene expression data using quantitative real time PCR (RT-qPCR). Since the underlying assumption of reference genes is that expressed at the exact same level in all sample types, in this study, we evaluated the expression stability of nine most commonly used endogenous controls (GAPDH, ACTB, 18S rRNA, RPS18, HSP-90, ALAS, HMBS, ACAC, and B2M) in four different tissues of the domestic goat, Capra hircus, including liver, visceral, subcutaneous fat and longissimus muscles, across different experimental treatments (a standard diet prepared using the NRC computer software as control and the same diet plus one mg chromium/day). We used six different

SUBMITTER: Najafpanah MJ 

PROVIDER: S-EPMC3865150 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature