Quantifying transient 3D dynamical phenomena of single mRNA particles in live yeast cell measurements.

ABSTRACT: Single-particle tracking (SPT) has been extensively used to obtain information about diffusion and directed motion in a wide range of biological applications. Recently, new methods have appeared for obtaining precise (10s of nm) spatial information in three dimensions (3D) with high temporal resolution (measurements obtained every 4 ms), which promise to more accurately sense the true dynamical behavior in the natural 3D cellular environment. Despite the quantitative 3D tracking information, the range of mathematical methods for extracting information about the underlying system has been limited mostly to mean-squared displacement analysis and other techniques not accounting for complex 3D kinetic interactions. There is a great need for new analysis tools aiming to more fully extract the b

SUBMITTER: Calderon CP 

PROVIDER: S-EPMC3865222 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature