Dynamic phase imaging utilizing a 4-dimensional microscope system.

ABSTRACT: This paper describes a new, novel interference Linnik microscope system and presents images and data of live biological samples. The specially designed optical system enables instantaneous 4-dimensional video measurements of dynamic motions within and among live cells without the need for contrast agents. This "label-free", vibration insensitive imaging system enables measurement of biological objects in reflection using harmless light levels with a variety of magnifications and wavelengths with fields of view from several hundred microns up to a millimeter. At the core of the instrument is a phase measurement camera (PMC) enabling simultaneous measurement of multiple interference patterns utilizing a pixelated phase mask taking advantage of the polarization properties of light. Utilizing

SUBMITTER: Creath K 

PROVIDER: S-EPMC3865252 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature