A universal method for species identification of mammals utilizing next generation sequencing for the analysis of DNA mixtures.

ABSTRACT: Species identification can be interesting in a wide range of areas, for example, in forensic applications, food monitoring and in archeology. The vast majority of existing DNA typing methods developed for species determination, mainly focuses on a single species source. There are, however, many instances where all species from mixed sources need to be determined, even when the species in minority constitutes less than 1 % of the sample. The introduction of next generation sequencing opens new possibilities for such challenging samples. In this study we present a universal deep sequencing method using 454 GS Junior sequencing of a target on the mitochondrial gene 16S rRNA. The method was designed through phylogenetic analyses of DNA reference sequences from more than 300 mammal species. Exp

SUBMITTER: Tillmar AO 

PROVIDER: S-EPMC3865308 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature