Active and secreted IgA-coated bacterial fractions from the human gut reveal an under-represented microbiota core.

ABSTRACT: Host-associated microbiota varies in distribution depending on the body area inhabited. Gut microbes are known to interact with the human immune system, maintaining gut homoeostasis. Thus, we studied whether secreted-IgA (S-IgA) coat specific microbial taxa without inducing strong immune responses. To do so, we fractionated gut microbiota by flow cytometry. We found that active and S-IgA-coated bacterial fractions were characterized by a higher diversity than those observed in raw faecal suspensions. A long-tail effect was observed in family distribution, revealing that rare bacteria represent up to 20% of total diversity. While Firmicutes was the most abundant phylum, the majority of its sequences were not assigned at the genus level. Finally, the single-cell-based approach enabled us to

SUBMITTER: D'Auria G 

PROVIDER: S-EPMC3865468 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature