X-ray solution scattering of squid heavy meromyosin: strengthening the evidence for an ancient compact off state.

ABSTRACT: The overall conformations of regulated myosins or heavy meromyosins from chicken/turkey, scallop, tarantula, limulus, and scorpion sources have been studied by a number of techniques, including electron microscopy, sedimentation, and pulsed electron paramagnetic resonance. These studies have indicated that the binding of regulatory ions changes the conformation of the molecule from a compact shape found in the "off" state of the muscle to extended relationships between the tail and independently mobile heads that predominate in the "on" state. Here we strengthen the argument for the generality of this conformational change by using small angle X-ray scattering on heavy meromyosin from squid. Small angle X-ray scattering allows the protein to be visualized in solution under mild and relativ

SUBMITTER: Gillilan RE 

PROVIDER: S-EPMC3866118 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature