Establishment of a bortezomib-resistant Chinese human multiple myeloma cell line: MMLAL.

ABSTRACT:

Background

A new human myeloma cell line, MMLAL, was established from the myelomatous pleural effusion of a 73-year-old Chinese patient suffering from symptomatic International stage III IgG/lambda myeloma. After a brief period of complete remission, he developed aggressive systemic relapse complicated by malignant pleural effusion with exclusive plasma cell infiltration. His disease remained chemo-refractory, and died six months after relapse.

Methods

Purified mononuclear cells from the pleural effusion of the patient were cultured in the presence of IL-6. Continually growing cells were characterized by morphological, immunophenotypic, cytogenetic, fluorescence in situ hybridization (FISH) and TP53 mutation analyses. Cell proliferation was measured and compared with other m

SUBMITTER: Wong KY 

PROVIDER: S-EPMC3866931 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature