SNPs affecting serum metabolomic traits may regulate gene transcription and lipid accumulation in the liver.

ABSTRACT:

Objective

Circulatory metabolites are important biomarkers for many diseases, especially metabolic disorders. The biological mechanism regulating circulatory levels of metabolites remains incompletely understood. Focusing on the liver as the central organ controlling metabolic homeostasis, we investigated the potential function of nine polymorphisms associated with serum metabolomic traits in a recent GWAS.

Materials/methods

The mRNA levels of the associated genes were measured by real-time PCR and correlated with genotypes in normal liver tissue (n=42). Genotype and mRNA data were also correlated with total hepatic lipid content (HLC). Our findings were also compared with the previously published gene expression quantitative traits loci (eQTL) data in the liver.

Results<

SUBMITTER: Mirkov S 

PROVIDER: S-EPMC3867007 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature