Potential-assisted adsorption of bovine serum albumin onto optically transparent carbon electrodes.

ABSTRACT: This article describes the effect of the applied potential on the adsorption of bovine serum albumin (BSA) to optically transparent carbon electrodes (OTCE). To decouple the effect of the applied potential from the high affinity of the protein for the bare surface, the surface of the OTCE was initially saturated with a layer of BSA. Experiments described in the article show that potential values higher than +500 mV induced a secondary adsorption process (not observed at open-circuit potential), yielding significant changes in the thickness (and adsorbed amount) of the BSA layer obtained. Although the process showed a significant dependence on the experimental conditions selected, the application of higher potentials, selection of pH values around the isoelectric point (IEP) of the protein,

SUBMITTER: Benavidez TE 

PROVIDER: S-EPMC3867293 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature