Electron capture dissociation of divalent metal-adducted sulfated N-glycans released from bovine thyroid stimulating hormone.

ABSTRACT: Sulfated N-glycans released from bovine thyroid stimulating hormone (bTSH) were ionized with the divalent metal cations Ca(2+), Mg(2+), and Co by electrospray ionization (ESI). These metal-adducted species were subjected to infrared multiphoton dissociation (IRMPD) and electron capture dissociation (ECD) and the corresponding fragmentation patterns were compared. IRMPD generated extensive glycosidic and cross-ring cleavages, but most product ions suffered from sulfonate loss. Internal fragments were also observed, which complicated the spectra. ECD provided complementary structural information compared with IRMPD, and all observed product ions retained the sulfonate group, allowing sulfonate localization. To our knowledge, this work represents the first application of ECD towards metal-add

SUBMITTER: Zhou W 

PROVIDER: S-EPMC3867818 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature