Novel ex vivo culture method for human monocytes uses shear flow to prevent total loss of transendothelial diapedesis function.

ABSTRACT: Monocyte recruitment to inflammatory sites and their transendothelial migration into tissues are critical to homeostasis and pathogenesis of chronic inflammatory diseases. However, even short-term suspension culture of primary human monocytes leads to phenotypic changes. In this study, we characterize the functional effects of ex vivo monocyte culture on the steps involved in monocyte transendothelial migration. Our data demonstrate that monocyte diapedesis is impaired by as little as 4 h culture, and the locomotion step is subsequently compromised. After 16 h in culture, monocyte diapedesis is irreversibly reduced by ∼90%. However, maintenance of monocytes under conditions mimicking physiological flow (5-7.5 dyn/cm²) is sufficient to reduce diapedesis impairment significantly. Thus, throu

SUBMITTER: Tsubota Y 

PROVIDER: S-EPMC3868191 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature