Genome organization and the role of centromeres in evolution of the erythroleukaemia cell line HEL.

ABSTRACT:

Background and objectives

The human erythroleukaemia (HEL) cell line has a highly rearranged genome. We matched whole chromosome analysis with cytogenomic microarray data to build a detailed description of these rearrangements.

Methodology

We used a combination of single nucleotide polymorphism array and multiple fluorescence in situ hybridization approaches, and compared our array data with publicly available data for different sublines of HEL. B allele frequencies revealed the fate of each homologue for most chromosomes.

Results

At least two instances of the breakage-fusion-bridge cycle appear to have facilitated amplification of oncogenes and deletion of tumour suppressor genes. Because our study included centromere identification, we found that some abnormal chrom

SUBMITTER: Mackinnon RN 

PROVIDER: S-EPMC3868402 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature