Evidence for gene duplication in the voltage-gated sodium channel gene of Aedes aegypti.

ABSTRACT:

Background and objectives

Mutations in the voltage-gated sodium channel gene (NaV), known as kdr mutations, are associated with pyrethroid and DDT insecticide resistance in a number of species. In the mosquito dengue vector Aedes aegypti, besides kdr, other polymorphisms allowed grouping AaNaV sequences as type 'A' or 'B'. Here, we point a series of evidences that these polymorphisms are actually involved in a gene duplication event.

Methodology

Four series of methods were employed: (i) genotypying, with allele-specific PCR (AS-PCR), of two AaNaV sites that can harbor kdr mutations (Ile1011Met and Val1016Ile), (ii) cloning and sequencing of part of the AaNaV gene, (iii) crosses with specific lineages and analysis of the offspring genotypes and (iv) copy number variation assa

SUBMITTER: Martins AJ 

PROVIDER: S-EPMC3868448 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature