Measurement of DNA concentration as a normalization strategy for metabolomic data from adherent cell lines.

ABSTRACT: Metabolomics is a rapidly advancing field, and much of our understanding of the subject has come from research on cell lines. However, the results and interpretation of such studies depend on appropriate normalization of the data; ineffective or poorly chosen normalization methods can lead to frankly erroneous conclusions. That is a recurrent challenge because robust, reliable methods for normalization of data from cells have not been established. In this study, we have compared several methods for normalization of metabolomic data from cell extracts. Total protein concentration, cell count, and DNA concentration exhibited strong linear correlations with seeded cell number, but DNA concentration was found to be the most generally useful method for the following reasons: (1) DNA concentrati

SUBMITTER: Silva LP 

PROVIDER: S-EPMC3868625 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature