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Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I.

ABSTRACT: A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal?I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.

SUBMITTER: Mbua NE 

PROVIDER: S-EPMC3869382 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I.

Mbua Ngalle Eric NE   Li Xiuru X   Flanagan-Steet Heather R HR   Meng Lu L   Aoki Kazuhiro K   Moremen Kelley W KW   Wolfert Margreet A MA   Steet Richard R   Boons Geert-Jan GJ  

Angewandte Chemie (International ed. in English) 20131015 49

A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease. ...[more]

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