Delivery of recombinant adeno-associated virus vectors to rat diaphragm muscle via direct intramuscular injection.

ABSTRACT: The diaphragm is the most important inspiratory muscle in all mammals, and ventilatory insufficiency caused by diaphragm dysfunction is the leading cause of morbidity and mortality in many genetic and acquired diseases affecting skeletal muscle. Currently, pharmacological inhibitors, genetically modified animals, and invasive procedures are used to study disorders affecting the diaphragm. However, these methodologies can be problematic because of off-target drug effects and the possible nonphysiological consequences of lifelong genetic alterations. Therefore, alternative methods to study this important respiratory muscle are needed. To resolve this, we have developed a methodology to deliver recombinant adeno-associated virus (rAAV) vectors to the rat diaphragm via direct intramuscular inj

SUBMITTER: Smuder AJ 

PROVIDER: S-EPMC3869534 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature