DNA barcoding and species boundary delimitation of selected species of Chinese Acridoidea (Orthoptera: Caelifera).

ABSTRACT: We tested the performance of DNA barcoding in Acridoidea and attempted to solve species boundary delimitation problems in selected groups using COI barcodes. Three analysis methods were applied to reconstruct the phylogeny. K2P distances were used to assess the overlap range between intraspecific variation and interspecific divergence. "Best match (BM)", "best close match (BCM)", "all species barcodes (ASB)" and "back-propagation neural networks (BP-based method)" were utilized to test the success rate of species identification. Phylogenetic species concept and network analysis were employed to delimitate the species boundary in eight selected species groups. The results demonstrated that the COI barcode region performed better in phylogenetic reconstruction at genus and species levels tha

SUBMITTER: Huang J 

PROVIDER: S-EPMC3869712 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature