Dynamics in cryo EM reconstructions visualized with maximum-likelihood derived variance maps.

ABSTRACT: CryoEM data capture the dynamic character associated with biological macromolecular assemblies by preserving the various conformations of the individual specimens at the moment of flash freezing. Regions of high variation in the data set are apparent in the image reconstruction due to the poor density that results from the lack of superposition of these regions. These observations are qualitative and, to date, only preliminary efforts have been made to quantitate the heterogeneity in the ensemble of particles that are individually imaged. We developed and tested a quantitative method for simultaneously computing a reconstruction of the particle and a map of the space-varying heterogeneity of the particle based on an entire data set. The method uses a maximum likelihood algorithm that expli

SUBMITTER: Wang Q 

PROVIDER: S-EPMC3870017 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature