Human dopamine transporter gene: differential regulation of 18-kb haplotypes.

ABSTRACT:

Aim

Since previous functional studies of short haplotypes and polymorphic sites of SLC6A3 have shown variant-dependent and drug-sensitive promoter activity, this study aimed to understand whether a large SLC6A3 regulatory region, containing these small haplotypes and polymorphic sites, can display haplotype-dependent promoter activity in a drug-sensitive and pathway-related manner.

Materials & methods

By creating and using a single copy number luciferase-reporter vector, we examined regulation of two different SLC6A3 haplotypes (A and B) of the 5´ 18-kb promoter and two known downstream regulatory variable number tandem repeats by 17 drugs in four different cellular models.

Results

The two regulatory haplotypes displayed up to 3.2-fold difference in promoter activity.

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC3870138 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature