The histone code of Toxoplasma gondii comprises conserved and unique posttranslational modifications.

ABSTRACT:

Unlabelled

Epigenetic gene regulation has emerged as a major mechanism for gene regulation in all eukaryotes. Histones are small, basic proteins that constitute the major protein component of chromatin, and posttranslational modifications (PTM) of histones are essential for epigenetic gene regulation. The different combinations of histone PTM form the histone code for an organism, marking functional units of chromatin that recruit macromolecular complexes that govern chromatin structure and regulate gene expression. To characterize the repertoire of Toxoplasma gondii histone PTM, we enriched histones using standard acid extraction protocols and analyzed them with several complementary middle-down and bottom-up proteomic approaches with the high-resolution Orbitrap mass spectrometer

SUBMITTER: Nardelli SC 

PROVIDER: S-EPMC3870261 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature