AGG/CCT interruptions affect nucleosome formation and positioning of healthy-length CGG/CCG triplet repeats.

ABSTRACT:

Background

Fragile X Syndrome (FXS), the most common inherited form of mental retardation, is caused by expansion of a CGG/CCG repeat tract in the 5'-untranslated region of the fragile X mental retardation (FMR1) gene, which changes the functional organization of the gene from euchromatin to heterochromatin. Interestingly, healthy-length repeat tracts possess AGG/CCT interruptions every 9-10 repeats, and clinical data shows that loss of these interruptions is linked to expansion of the repeat tract to disease-length. Thus, it is important to understand how these interruptions alter the behavior of the repeat tract in the packaged gene.

Results

To investigate how uninterrupted and interrupted CGG/CCG repeat tracts interact with the histone core, we designed experiments using

SUBMITTER: Volle CB 

PROVIDER: S-EPMC3870987 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature