Describing autophagy via analysis of individual organelles by capillary electrophoresis with laser induced fluorescence detection.

ABSTRACT: Autophagy is a cellular process responsible for the degradation of intracellular cargo. Its dynamic nature and the multiple types of autophagy organelles present at a given time make current measurements, such as those done by Western blotting, insufficient to understand autophagy and its roles in aging and disease. Capillary electrophoresis coupled to laser induced fluorescence detection (CE-LIF) has been used previously to count and determine properties of individual organelles, but has never been used on autophagy organelles or for determination of changes of such properties. Here we used autophagy organelles isolated from L6 cells expressing GFP-LC3, which is an autophagy marker, to develop a CE-LIF method for the determination of the number of autophagy organelles, their individual GF

SUBMITTER: Satori CP 

PROVIDER: S-EPMC3872064 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature